Histopathological And Biochemical Changes In Larvae Of Channa Punctatus Exposed To Thifluzamide

Abhimanyu Singh

Dr. Rittika Pandey

Dr. Anupma Kumari

Dr. Nirmala Tripathi

Vol. 9, Issue 1, Jan-Dec 2023

Page Number: 72 - 85

Abstract:

This study examines the histological and biochemical alterations in Channa punctatus larvae (spotted snakehead) subjected to different thifluzamide dosages. Several well-controlled lab investigations evaluated the effects on the liver, gills, and kidneys. Enzyme activity and protein levels in these organs were also investigated. Our experiment Channa punctatus larvae to different thifluzamide doses throughout time. Histopathology revealed cellular disintegration, tissue necrosis, and significant structural changes in vital organs such the liver, gills, and kidneys. Thorough liver tissue examination revealed vacuolation and hepatocellular deterioration. However, microscopic gill investigation showed lamellar fusion and epithelial elevation. Renal tissues exhibited extensive glomerular atrophy and tubular degeneration, suggesting renal function loss. The biochemical examination showed considerable enzyme activity and protein alterations, indicating biological system problems. ALT and AST levels increased significantly, indicating hepatic stress and liver failure. Furthermore, antioxidant enzymes like SOD and CAT showed changed activity, indicating oxidative stress responses. Tissue protein levels fluctuated throughout the study, suggesting disruptions in complex metabolic and protein synthesis networks that control cellular function. Importantly, thifluzamide may affect Channa punctatus larvae's growth and survival, according to studies. aquatic environment fungicide contamination ecological impacts. Histological and biochemical changes in fish species after extended thifluzamide exposure indicate that critical physiological processes are adversely impacted. These fascinating findings demonstrate long-term damage to diverse fish populations and aquatic biodiversity. This study underlines the necessity to closely control and monitor agricultural chemical products to decrease their environmental effect and conserve aquatic ecosystems' biodiversity.

References

• Khatun, A., Jahan, S., & Rahman, M. M. (2016). Histopathological effects of pesticides on fish. Journal of Applied Environmental and Biological Sciences, 6(5), 28-33.
• Pandey, S., Kumar, R., Sharma, S., Nagpure, N. S., Srivastava, S. K., & Verma, M. S. (2005). Acute toxicity bioassay of mercuric chloride and malathion on air-breathing fish Channa punctatus (Bloch). Ecotoxicology and Environmental Safety, 61(1), 114-120.
• Ghosh, A., & Mukherjee, D. (2013). Histopathological and biochemical changes in fish liver due to pesticides. Environmental Toxicology and Pharmacology, 36(2), 625-634.
• Tripathi, G., & Verma, P. (2004). Endosulfan-mediated biochemical changes in the freshwater fish Clarias batrachus. Journal of Environmental Biology, 25(4), 323-329.
• Authman, M. M. N., Abbas, H. H., & Abbas, W. T. (2015). Assessment of DNA damage in freshwater fish, Nile tilapia (Oreochromis niloticus), exposed to pesticides. Aquatic Toxicology, 162, 78-86.
• Velisek, J., Svobodova, Z., & Piackova, V. (2009). Effects of acute exposure to deltamethrin on some haematological, biochemical and histopathological parameters of rainbow trout (Oncorhynchus mykiss). Veterinary Medicine, 54(3), 131-137.
• Ansari, B. A., & Kumar, K. (1988). Malathion toxicity: Effect on the liver of a freshwater teleost, Channa punctatus (Bloch). Bulletin of Environmental Contamination and Toxicology, 41(5), 822-826.
• Agrahari, S., Gopal, K., & Pandey, K. C. (2006). Biomarkers of monocrotophos toxicity to fish. Indian Journal of Experimental Biology, 44(10), 890-897.
• Mahboob, S., Al-Balawi, H. F., Al-Misned, F., Ahmad, Z., & Sultana, S. (2014). Investigation on the effects of pesticides on the liver function of fish: a review. Journal of Aquatic Biology & Fisheries, 2(1), 15-23.
• Jha, A. N., & Hossain, M. A. (2008). Pesticide-induced DNA damage in fish. Aquatic Toxicology, 86(1), 132-138.
• Xie, L., Liu, Y., Zhang, Y., Wang, X., & Zhang, Y. (2016). Hepatic damage and antioxidant enzyme responses in zebrafish exposed to fungicides. Environmental Toxicology and Pharmacology, 45, 64-70. https://doi.org/10.1016/j.etap.2016.06.004
• Ghisi, N. de C., & Cestari, M. M. (2011). Genotoxic effects of the fungicide tebuconazole in two fish species from Southern Brazil: A case study in a rice paddy field. Ecotoxicology and Environmental Safety, 74(4), 832-839. https://doi.org/10.1016/j.ecoenv.2011.07.013
• Guilhermino, L., Diamantino, T., Silva, M. C., & Soares, A. M. (2000). Acute toxicity test with Daphnia magna: An alternative to mammals in the pre-screening of chemical toxicity? Ecotoxicology and Environmental Safety, 46(3), 357-362. https://doi.org/10.1006/eesa.2000.1912
• Zhao, X., & Zhang, X. (2017). Subchronic exposure to pyraclostrobin induces liver damage and oxidative stress in common carp. Environmental Pollution, 229, 354-362. https://doi.org/10.1016/j.envpol.2017.02.004
• Li, Z., & Liu, Q. (2019). Oxidative stress and antioxidant defense responses in fish exposed to various concentrations of atrazine. Aquatic Toxicology, 210, 208-216. https://doi.org/10.1016/j.aquatox.2019.03.006
• Ananth, S., & Thirumurugan, R. (2014). Toxicological evaluation and antioxidant response of freshwater fish exposed to a pesticide mixture. Ecotoxicology and Environmental Safety, 106, 28-34. https://doi.org/10.1016/j.ecoenv.2014.07.018
• Santos, L. H. M. L. M., Araújo, A. N., Fachini, A., Pena, A., Delerue-Matos, C., & Montenegro, M. C. B. S. M. (2018). Ecotoxicological aspects related to the presence of pharmaceuticals in the aquatic environment. Journal of Hazardous Materials, 358, 236-258. https://doi.org/10.1016/j.jhazmat.2017.12.123
• Glusczak, L., Miron, D. dos S., Moraes, B. S., Simões, R. R., Schetinger, M. R. C., & Morsch, V. M. (2007). Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(4), 519-524. https://doi.org/10.1016/j.cbpc.2007.06.004
• Wiegand, C., Krause, E., Steinberg, C., & Pflugmacher, S. (2001). Toxicokinetic of atrazine in embryos of the zebrafish (Danio rerio). Ecotoxicology and Environmental Safety, 49(3), 199-205. https://doi.org/10.1006/eesa.2001.2065
• Livingstone, D. R. (2001). Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Marine Pollution Bulletin, 42(8), 656-666. https://doi.org/10.1016/S0025- 326X(01)00060-1
• Halliwell, B., & Gutteridge, J. M. (1984). Oxygen toxicity, oxygen radicals, transition metals and disease. Biochemical Journal, 219(1), 1-14. https://doi.org/10.1042/bj2190001
• Halliwell, B., & Gutteridge, J. M. (1990). The antioxidants of human extracellular fluids. Archives of Biochemistry and Biophysics, 280(1), 1-8. https://doi.org/10.1016/0003-9861(90)90510-P
• Valko, M., Rhodes, C. J., Moncol, J., Izakovic, M., & Mazur, M. (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions, 160(1), 1-40. https://doi.org/10.1016/j.cbi.2005.12.009

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